What are the recommended research applications for the Mouse Anti-VEGF Recombinant Antibody (clone A4.6.1)?

This antibody is highly suited for a variety of laboratory techniques such as Neutralization, ELISA, Immunocytochemistry (ICC), and Western Blot (WB) applications. It allows researchers to examine VEGF levels and investigate its role in processes like angiogenesis, which is particularly relevant in cancer research and vascular biology studies.

What are the storage and handling recommendations for maintaining the stability of this antibody?

For optimal stability, store the antibody at +4°C for short-term (1-2 weeks) and at -20°C for long-term storage. It's important to aliquot the antibody upon first receipt to avoid repeated freeze-thaw cycles, which could adversely affect its performance.

Mouse Anti-VEGF Recombinant Antibody (clone A4.6.1)

CAT#: PABW-130

Recombinant Mouse Antibody (A4. 6. 1) is capable of binding to VEGF, expressed in Chinese Hamster Ovary cells (CHO).

Published Data Tested Data

Inhib

Figure 1 In vivo potency and efficacy of anti-VEGF antibodies in inhibiting HM-7 (human colorectal) xenografts.

A, dose-responsive inhibition of tumor growth by A4.6.1, Y0317, B20-4.1, and G6-31 (5, 0.5, and 0.1 mg/kg, twice weekly) in HM-7 bearing mouse was shown by the tumor size (mm³) as a function of time (days). Error bars represent the S.E. (n = 10).

Liang, W. C., Wu, X., Peale, F. V., Lee, C. V., Meng, Y. G., Gutierrez, J., ... & Fuh, G. (2006). Cross-species vascular endothelial growth factor (VEGF)-blocking antibodies completely inhibit the growth of human tumor xenografts and measure the contribution of stromal VEGF. Journal of Biological Chemistry, 281(2), 951-961.

ICC

Figure 2 Examination of tumor sections.

B, medium magnification images (bar = 500 μm) of pancreatic HPAC (a, e, and i) and colonic HM-7 (b, f, and j) xenografts treated with control antibody (a and b), anti-human VEGF, Avastin (A4.6.1 for HM-7) (e and f), or anti-mouse/human VEGF, G6-31 (i and j). All images are centered 1 mm from the tumor/host margin. High magnification images (bar = 50 μm) of HPAC (c, g, and k) and HM-7 (d, h, and l) tumor xenografts treated with control antibody (c and d), anti-human VEGF, Avastin (g and h), or anti-mouse/human VEGF, G6-31 (k and l). Compared with HM-7, HPAC xenografts are relatively stromarich (compare c and d). Control antibody-treated HPAC tumors have extensive stromal cells infiltration (arrowheads, c), with indistinct small-caliber vessels surrounded by connective tissue cells and matrix. Treatment of these tumors with anti-human (g) or anti-mouse/human VEGF (k) results in little change in tumor-stroma proportion. In contrast, control HM-7 tumors (d) have relatively sparse stroma, and large caliber thin walled vessels (arrow). Treatment with anti-human VEGF (h) reduces vessel diameter and increases the relative amount of stromal matrix and cells around remaining vessels. Similar but more dramatic changes occur when HM-7 tumors are treated with anti-mouse/human VEGF, G6-31 (l). Images c, d, g, h, and k are centered 1 mm from the tumor/host margin (left in the images); image l is centered 200 μm from the tumor/host margin (in this sample, only necrotic tumor is present at 1 mm from the host margin).

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Figure 1 Mouse Anti-VEGF Recombinant Antibody (PABW-130) in WB

Western blot analysis was performed with 0.1 µg (lane 1), 0.3 µg(lane 2), and 0.6 µg (lane 3) recombinant human VEGF onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with PABW-130 at 2ng/μL overnight at 4°C on a rocking platform. Then probed with Goat anti-Mouse IgG HRP secondary antibody at a dilution of 1:3,000 for one hour. Chemiluminescent was detected.

Figure 2 Mouse Anti-VEGF Recombinant Antibody (PABW-130) in Dot Blot

Dot Blot analysis of PABW-130 was performed by coating with human VEGF antigen.
PABW-130 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)

ELISA

Figure 3 Mouse Anti-VEGF Recombinant Antibody (PABW-130) in ELISA

ELISA analysis of PABW-130 was performed by coating with recombinant human VEGF antigen. Then blocked with BSA, and incubated with PABW-130 at a starting concentration of 2.5 µg/mL. The HRP-conjugated Goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

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Specifications

Immunogen
Human Vascular endothelial growth factor

Host Species
Mouse

Type
Mouse IgG

Specificity
Human VEGF

Species Reactivity
Human

Clone
A4.6.1

Applications
Neut, ELISA, Inhib, ICC, WB

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The VEGF antibody has been reported in applications of Neutralization, Enzyme-linked Immunosorbent Assay, Inhibition, Immunocytochemistry, Western Blot.

Target

Alternative Names
Vascular endothelial growth factor; VEGF

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Breast cancer biomarkers at key points during disease progression

Versatility for Various Applications

As a researcher delving into vascular biology, I've found this antibody particularly versatile for applications like ELISA and ICC. It stands out due to its high specificity and purity, ensuring that I get precise results no matter the application. Such versatility makes it a staple in my research toolkit.

Reliable for VEGF Pathway Studies

This antibody has exceeded expectations with its performance in VEGF pathways studies. The ease of use and the clear distinction it provides in protein analysis using Western Blot techniques make this product a true asset. It's further enhanced by the robust support offered by Creative Biolabs.

Q&As

What are the recommended research applications for the Mouse Anti-VEGF Recombinant Antibody (clone A4.6.1)?

A: This antibody is highly suited for a variety of laboratory techniques such as Neutralization, ELISA, Immunocytochemistry (ICC), and Western Blot (WB) applications. It allows researchers to examine VEGF levels and investigate its role in processes like angiogenesis, which is particularly relevant in cancer research and vascular biology studies.
What are the storage and handling recommendations for maintaining the stability of this antibody?

A: For optimal stability, store the antibody at +4°C for short-term (1-2 weeks) and at -20°C for long-term storage. It's important to aliquot the antibody upon first receipt to avoid repeated freeze-thaw cycles, which could adversely affect its performance.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABW-130, RRID: AB_3111686)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABW-130.pdf

MSDS

MSDS - PABW-130.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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